Green Stability Indicating Organic Solvent-Free HPLC Determination of Remdesivir in Substances and Pharmaceutical Dosage Forms

A green liquid chromatographic method is considered in this work to minimize the environmental impact of waste solvents. One important principle replace or eliminate use hazardous organic Organic impurities any active pharmaceutical ingredient could arise either during process its synthesis, as degradation products developed throughout shelf-life. Remdesivir (RDS) an antiviral drug, approved by US Food and Drug Adminstration (-FDA), treat SARS-Cov-2 virus pandemic crisis. We studied stability remdesivir against several pathways using solvent-free technique. Separation was performed on RP-C18 stationary phase mixed-micellar mobile composed a mixture 0.025 M Brij-35, 0.1 sodium lauryl sulfate (SLS), 0.02 disodium hydrogen phosphate, adjusted pH 6.0. The flow rate 1 mL min−1, detection carried out at wavelength 244 nm. profiled that originated mild drastic conditions. then validated according International Conference Harmonization (ICH) guidelines within linearity range 5–100 μg mL−1 applied successfully for determination drug marketed dosage form. brief comparison established with reported methods, including greenness assessment two new metrics (GAPI AGREE). This study first be eco-friendly, solvent-free, indicating LC methodology RDS impurity profiling.